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Interestingly, adult mice with modest isolated elevations in GH and IGF-1 levels have greater lean mass, improved lipid oxidation and glucose clearance 38 , while specific, temporal reductions in GH and IGF-1 in adults have been linked to deleterious metabolic effects, including impaired hepatic insulin action 38 , and reduced -cell mass and function 39

This shift in perspective also opens new challenges for computational and experimental drug discovery

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The enzymatic reaction was performed at 37 C for 30 min and terminated by adding 0.4 mL of 4 M acetic acid